other:
Immunofluorescence:Article Title: Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression
Article Snippet: .. To identify the characteristics of hNSCs and hOPCs, the cells were seeded in 24-well plates and immunofluorescence staining was performed using mouse anti-A2B5 (1:50; Cat. #MAB1416, R&D Systems, Minneapolis, MN, USA, RRID: AB_357687 ), rabbit anti-platelet-derived growth factor (PDGF) receptor-α (1:800; Cat. #C5241, Cell Signaling Technology, Boston, MA, USA, RRID: AB_10692773 ), rabbit anti-oligodendrocyte transcription factor 2 (OLIG2) (1:300; Cat. #AB9610, Millipore, MA, USA, RRID: AB_570666 ), mouse anti-sex-determining region Y (SRY)-box transcription factor 10 (SOX10) (1:300; Cat. #MAB2864, R&D Systems, Minneapolis, MN, USA, RRID: AB_2195180 ), and mouse anti-nestin (1:100; Cat. #ab6320, Abcam, Cambridge, UK, RRID: AB_308832 ). .. Flow cytometric analysis was performed using BV421 PDGFR-α mouse anti-human (Cat. #562799, BD Biosciences, Franklin Lake, NJ, USA, RRID: AB_2737804 ) and PE A2B5 mouse anti-human (Cat. #130-093-581, Miltenyi Biotec, Bergisch-Gladbach, Germany, RRID: AB_10830711 ). hOPCs-1 (cells prepared for desensitization), hNSCs-1 (hOPCs-1 differentiated from hNSCs-1), and hOPCs-2 (hOPCs-1 and hNSCs-1 derived from the same fetus whereas hOPCs-2 derived from the other fetus) were respectively included in our study which were digested and resuspended in saline at 4 °C.
Article Title: Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression.
Article Snippet: .. To identify the characteristics of hNSCs and hOPCs, the cells were seeded in 24-well plates and immunofluorescence staining was performed using mouse anti-A2B5 (1:50; Cat. #MAB1416, R&D Systems, Minneapolis, MN, USA, RRID: AB_357687), rabbit anti-platelet-derived growth factor (PDGF) receptor-a (1:800; Cat. #C5241, Cell Signaling Technology, Boston, MA, USA, RRID: AB_10692773), rabbit anti-oligodendrocyte transcription factor 2 (OLIG2) (1:300; Cat. #AB9610, Millipore, MA, USA, RRID: AB_570666), mouse antisex-determining region Y (SRY)-box transcription factor 10 (SOX10) (1:300; Cat. #MAB2864, R&D Systems, Minneapolis, MN, USA, RRID: AB_2195180), and mouse anti-nestin (1:100; Cat. #ab6320, Abcam, Cambridge, UK, RRID: AB_308832). .. Flow cytometric analysis was performed using BV421 PDGFR-a mouse anti-human (Cat. #562799, BD Biosciences, Franklin Lake, NJ, USA, RRID: AB_2737804) and PE A2B5 mouse anti-human (Cat. #130-093-581, Miltenyi Biotec, Bergisch-Gladbach, Germany, RRID: AB_10830711). hOPCs-1 (cells prepared for desensitization), hNSCs-1 (hOPCs-1 differentiated from hNSCs-1), and hOPCs-2 (hOPCs-1 and hNSCs-1 derived from the same fetus whereas hOPCs-2 derived from the other fetus) were respectively included in our study which were digested and resuspended in saline at 4 C. Ten of passage of hNSCs and three to five of passage of hOPCs were chosen in intrauterine desensitization and transplantation.
Staining:Article Title: Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression
Article Snippet: .. To identify the characteristics of hNSCs and hOPCs, the cells were seeded in 24-well plates and immunofluorescence staining was performed using mouse anti-A2B5 (1:50; Cat. #MAB1416, R&D Systems, Minneapolis, MN, USA, RRID: AB_357687 ), rabbit anti-platelet-derived growth factor (PDGF) receptor-α (1:800; Cat. #C5241, Cell Signaling Technology, Boston, MA, USA, RRID: AB_10692773 ), rabbit anti-oligodendrocyte transcription factor 2 (OLIG2) (1:300; Cat. #AB9610, Millipore, MA, USA, RRID: AB_570666 ), mouse anti-sex-determining region Y (SRY)-box transcription factor 10 (SOX10) (1:300; Cat. #MAB2864, R&D Systems, Minneapolis, MN, USA, RRID: AB_2195180 ), and mouse anti-nestin (1:100; Cat. #ab6320, Abcam, Cambridge, UK, RRID: AB_308832 ). .. Flow cytometric analysis was performed using BV421 PDGFR-α mouse anti-human (Cat. #562799, BD Biosciences, Franklin Lake, NJ, USA, RRID: AB_2737804 ) and PE A2B5 mouse anti-human (Cat. #130-093-581, Miltenyi Biotec, Bergisch-Gladbach, Germany, RRID: AB_10830711 ). hOPCs-1 (cells prepared for desensitization), hNSCs-1 (hOPCs-1 differentiated from hNSCs-1), and hOPCs-2 (hOPCs-1 and hNSCs-1 derived from the same fetus whereas hOPCs-2 derived from the other fetus) were respectively included in our study which were digested and resuspended in saline at 4 °C.
Article Title: Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression.
Article Snippet: .. To identify the characteristics of hNSCs and hOPCs, the cells were seeded in 24-well plates and immunofluorescence staining was performed using mouse anti-A2B5 (1:50; Cat. #MAB1416, R&D Systems, Minneapolis, MN, USA, RRID: AB_357687), rabbit anti-platelet-derived growth factor (PDGF) receptor-a (1:800; Cat. #C5241, Cell Signaling Technology, Boston, MA, USA, RRID: AB_10692773), rabbit anti-oligodendrocyte transcription factor 2 (OLIG2) (1:300; Cat. #AB9610, Millipore, MA, USA, RRID: AB_570666), mouse antisex-determining region Y (SRY)-box transcription factor 10 (SOX10) (1:300; Cat. #MAB2864, R&D Systems, Minneapolis, MN, USA, RRID: AB_2195180), and mouse anti-nestin (1:100; Cat. #ab6320, Abcam, Cambridge, UK, RRID: AB_308832). .. Flow cytometric analysis was performed using BV421 PDGFR-a mouse anti-human (Cat. #562799, BD Biosciences, Franklin Lake, NJ, USA, RRID: AB_2737804) and PE A2B5 mouse anti-human (Cat. #130-093-581, Miltenyi Biotec, Bergisch-Gladbach, Germany, RRID: AB_10830711). hOPCs-1 (cells prepared for desensitization), hNSCs-1 (hOPCs-1 differentiated from hNSCs-1), and hOPCs-2 (hOPCs-1 and hNSCs-1 derived from the same fetus whereas hOPCs-2 derived from the other fetus) were respectively included in our study which were digested and resuspended in saline at 4 C. Ten of passage of hNSCs and three to five of passage of hOPCs were chosen in intrauterine desensitization and transplantation.
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